The role of oxidative clustered DNA lesions during breast cell transformation / by Charles-Thomas D. Cooke.
| Author/creator | Cooke, Charles-Thomas D. author. |
| Other author | Sigounas, George, degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 2007. |
| Description | 89 leaves : illustrations ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Summary | Breast cancer is the most common non-skin cancer and the second leading cause of cancer-related death in women in the United States. There are several environmental and genetic risk factors for the disease, which may induce different types of DNA damage. Carcinogens, such as polycyclic aromatic hydrocarbons (PAHs), may play a role in the initiation of breast cancer. Benzo[a]pyrene (B[a]P) is one of the most widely studied PAHs and it has been shown to induce transformation of normal breast cells, which may be the result of gene alterations. This study hypothesizes that unstable DNA adducts and reactive oxygen species induce multiple lesions that are in close proximity to one another, termed Oxidative Clustered DNA Lesions (OCDLs). Normal early and late passage primary breast tissue-derived cells were treated with B[a]P and the levels of OCDLs and chromosomal aberrations were determined. This study found that: 1) there is an accumulation of various types of complex DNA damage, including OCDLs, in primary breast cells exposed to B[a]P; 2) after exposure to B[a]P, there is an increase in the number of chromosomal aberrations, which have been reported to be directly linked to genetic instability, mutagenesis and cancer; 3) there is a strong positive correlation between the number of OCDLs and the number of chromosomal aberrations. In conclusion, these results provide an indication that OCDLs may play a significant role during transformation of normal primary breast cells. |
| General note | Presented to the faculty of the Department of Biology. |
| General note | Advisor: George Sigounas |
| Dissertation note | M.S. East Carolina University 2007 |
| Bibliography note | Includes bibliographical references (leaves 82-89). |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |
Availability
| Library | Location | Call Number | Status | Item Actions |
|---|---|---|---|---|
| Joyner | University Archives | ASK AT SPECIAL COLLECTIONS DESK | ✔ Available | Request Material |
| Electronic Resources | Access Content Online | ✔ Available |