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003 OCoLC;

005 20260420152327.0;

008 071031s2006 xx a bm 000 0 eng d;

035 a| (Sirsi) o180100944;

035 a| (OCoLC)180100944;

049 a| EREE;

050 4 a| QR400b| .W45 2006;

100 1 a| Whitman, Audy G.,e| author.?| UNAUTHORIZED;

245 10 a| Changes occuring [sic] on the cell surface during KSHV reactivation /c| by Audy G. Whitman.;

246 3 a| Changes occurring on the cell surface during KSHV reactivation;

264 0 c| 2006.;

300 a| xi, 79 leaves :b| illustrations (some color) ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 2006;

500 a| Presented to the faculty of the Department of Biology.;

500 a| Advisor: Shaw M. Akula;

520 3 a| KSHV is an etiological agent of Kaposi's sarcoma, primary effusion lymphoma, and multicentric Castleman disease. KSHV infection and pathogenesis are common in certain regions of the world and in immunocompromised patients. Due to an upsurge in the worldwide population of immunocompromised patients from HIV/AIDS, organ transplant, and cancer treatment (chemo- and radiation-therapy), KSHV is becoming an increasing problem to the medical profession. Viral egress is a process in which a virus particle escapes a membrane of the host cell. Egress from the plasma membrane is critical for the successful spread of viral infection. After infection, KSHV exists predominantly in a dormant state, with only 1 -2% infected cells undergoing spontaneous reactivation to produce an active (lytic) infection. The exact mechanism that leads to KSHV reactivation is still elusive. Recent studies have demonstrated that S-phase of the cell cycle supports an active KSHV lytic reactivation. Hence, the goal of this study was to analyze KSHV reactivation process with a special emphasis to cell cycle progression and changes occurring on the cell surface. This was accomplished by the use of three different approaches: scanning electron microscopy, transmission electron microscopy, and biochemical analysis. Results from this study demonstrate for the first time, a direct link between cell cycle progression, cell surface topology, and KSHV reactivation. These findings are crucial for future advances in understanding the biology of KSHV and in developing anti-viral drugs to control KSHV associated pathogenesis. Further, we foresee this study to serve as a model to unravel the mysteries surrounding virus latency.;

504 a| Includes bibliographical references (leaves 49-65).;

650 0 a| Herpesviruses.=| ^A144272;

650 0 a| Kaposi's sarcoma.=| ^A708280;

650 0 a| Cell cycle.=| ^A4972;

650 0 a| Cell membranes.=| ^A1027;

650 7 a| Cell cycle.2| fast0| (OCoLC)fst00850178;

650 7 a| Cell membranes.2| fast0| (OCoLC)fst00850194;

650 7 a| Herpesviruses.2| fast0| (OCoLC)fst00955650;

650 7 a| Kaposi's sarcoma.2| fast0| (OCoLC)fst00986150;

655 7 a| dissertations.2| aat0| (CStmoGRI)aatgf300028029;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Akula, Shaw M.,e| degree supervisor.1| https://id.oclc.org/worldcat/entity/E39PCjKQf4c4xJJb73dbXvgWcK=| ^A1406820;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/12929;

949 a| Click on web addressw| asish| joyner101;

949 a| Click on web addressw| asish| hsl111;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 1271091;

999 a| ASK AT SPECIAL COLLECTIONS DESKw| ASISc| 1i| MQ2265977l| JSCJTHDm| JOYNERr| Ys| Yt| JSCJTHDu| 10/31/2007x| ETDz| JSPECCOLLo| .STAFF. 0;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 1271091-3001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 4/14/2026x| ETDz| JERESOURCE;

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Changes occuring [sic] on the cell surface during KSHV reactivation / by Audy G. Whitman.

Author/creator	Whitman, Audy G. author.
Other author	Akula, Shaw M., degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	2006.
Description	xi, 79 leaves : illustrations (some color) ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Herpesviruses. Kaposi's sarcoma. Cell cycle. Cell membranes.

Variant title	Changes occurring on the cell surface during KSHV reactivation
Summary	KSHV is an etiological agent of Kaposi's sarcoma, primary effusion lymphoma, and multicentric Castleman disease. KSHV infection and pathogenesis are common in certain regions of the world and in immunocompromised patients. Due to an upsurge in the worldwide population of immunocompromised patients from HIV/AIDS, organ transplant, and cancer treatment (chemo- and radiation-therapy), KSHV is becoming an increasing problem to the medical profession. Viral egress is a process in which a virus particle escapes a membrane of the host cell. Egress from the plasma membrane is critical for the successful spread of viral infection. After infection, KSHV exists predominantly in a dormant state, with only 1 -2% infected cells undergoing spontaneous reactivation to produce an active (lytic) infection. The exact mechanism that leads to KSHV reactivation is still elusive. Recent studies have demonstrated that S-phase of the cell cycle supports an active KSHV lytic reactivation. Hence, the goal of this study was to analyze KSHV reactivation process with a special emphasis to cell cycle progression and changes occurring on the cell surface. This was accomplished by the use of three different approaches: scanning electron microscopy, transmission electron microscopy, and biochemical analysis. Results from this study demonstrate for the first time, a direct link between cell cycle progression, cell surface topology, and KSHV reactivation. These findings are crucial for future advances in understanding the biology of KSHV and in developing anti-viral drugs to control KSHV associated pathogenesis. Further, we foresee this study to serve as a model to unravel the mysteries surrounding virus latency.
General note	Presented to the faculty of the Department of Biology.
General note	Advisor: Shaw M. Akula
Dissertation note	M.S. East Carolina University 2006
Bibliography note	Includes bibliographical references (leaves 49-65).
Genre/form	dissertations.
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

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