Characterization and expression of HOXb1b in Morone saxatilis / by Matthew H. Smith.
| Author/creator | Smith, Matthew H. author. |
| Other author | Scemama, Jean-Luc, degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 2004. |
| Description | 96 leaves : illustrations (some color) ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Summary | Hox genes are transcription factors that control patterning of body axes in bilaterians. These genes are found in clusters with a variable numbers of genes on each cluster that is species specific. The most 3' of these clustered genes are in paralogous group (PG) one. PGl genes function in the patterning of the hindbrain, specifically in specifying the identity of rhombomere (r) four. Expression of PGl Hox genes is controlled by three specific transcription elements; two retinoic acid response elements (RAREs) and one autoregulatory element. In Mus musculus, a RARE-DR2 element drives early expression of PGl Hox genes in r4. This r4 expression is later maintained by the Hoxbl autoregulatory element (Hoxbl-ARE) serving as a recognition site for Hoxbl/Pbx and other complexes. Another RARE element (RARE-DR5) drives expression in the foregut of Mus musculus. In this study, we identified a Hoxblb gene in Morone scaatilis and analyzed the expression pattern of that gene in the developing embryo. Unlike Danio rerio Hoxblb, Morone saxatilis Hoxblb lacks early expression in r4. These expression patterns were found to correlate with the regulatory element present on that cluster. Comparative genomic analysis between Morone scaatilis, Danio rerio, Takifugu rubripes, and Branchiostomafloridae showed that six RARE-DR elements were present in the common ancestor of Danio rerio and Takifugu rubripes which occurred in the euteleost lineage just after the emergence of the teleosts. The divergent lineages that these fish represent are hypothesized to have differentially lost redundant regulatory elements while preserving the overall function of the ancestral genes. Based on comparative analysis and the phylogenetic relationships between Morone saxatilis, Takifugu rubripes, and Danio rerio it is hypothesized that the organization of PGl genes in Morone saxatilis will be found to be more like that in Takifugu rubripes than in Danio rerio. |
| General note | Presented to the faculty of the Department of Biology. |
| General note | Advisor: Jean-Luc Scemama |
| Dissertation note | M.S. East Carolina University 2004 |
| Bibliography note | Includes bibliographical references (leaves 87-92). |
| Genre/form | dissertations. |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |
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|---|---|---|---|---|
| Joyner | University Archives | ASK AT SPECIAL COLLECTIONS DESK | ✔ Available | Request Material |
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