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035 a| (Sirsi) o71354526;

035 a| (OCoLC)71354526;

049 a| EREE;

050 4 a| QL950.7b| .S66 2004;

100 1 a| Snow, Holly Elizabeth,e| author.?| UNAUTHORIZED;

245 10 a| Versican expression during skeletal/joint morphogenesis and patterning of muscle and nerve in the embryonic mouse limb /c| by Holly Elizabeth Snow.;

264 0 c| 2004.;

300 a| 57 leaves :b| illustrations (some color) ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 2004;

500 a| Presented to the faculty of the Department of Biology.;

500 a| Advisor: Anthony A. Capehart;

504 a| Includes bibliographical references (leaves 46-57).;

520 3 a| Versican, an extracellular matrix proteoglycan, has been implicated in limb skeletogenesis and is localized in mesenchymal precartilage condensations. However, studies have not investigated spatial and temporal expression of versican and its relationship to muscle and nerve patterning during mammalian limb development. The heart defect {hdf) transgenic mouse has a lacZ reporter construct within the versican gene {hdf transgene), which provides a novel model by which to study the role of versican in limb development. In addition, histochemical localization of the lacZ reporter is a simple method for localization of transgene expression, Hdf transgene expression in whole mount heterozygous embryos and localization of versican relative to cartilage, muscle, and nerve tissues in paraffin-embedded limb sections of wild-type embryos from 10.5-14 dpc were evaluated by lacZ histochemistry and immunohistochemistry. Versican was localized within precartilage condensations of mesenchyme and nascent cartilage nodules, with expression diminishing upon maturation of the cartilage model. Interestingly, versican expression remained strong in developing synovial joint interzones, suggesting a potential function for versican in joint morphogenesis. Isolated myoblasts, incipient skeletal muscle masses, and neurites were absent in areas of strong versican expression within the developing limb. Versican expressing tissues may reserve space for the future limb skeleton and developing joints and may aid in patterning of muscle and nerve by deterring muscle migration and innervation into these regions.;

650 0 a| Extremities (Anatomy)=| ^A3514;

650 0 a| Proteoglycans.=| ^A14614;

650 0 a| Morphogenesis.=| ^A1884;

650 0 a| Mice as laboratory animals.=| ^A848;

650 2 a| Extremities.0| (DNLM)D005121=| ^A918870;

650 2 a| Proteoglycans.0| (DNLM)D011509=| ^A1473685;

655 2 a| Academic Dissertation.0| (DNLM)D019478?| UNAUTHORIZED;

650 7 a| Extremities (Anatomy)2| fast0| (OCoLC)fst00919071;

650 7 a| Mice as laboratory animals.2| fast0| (OCoLC)fst01019388;

650 7 a| Morphogenesis.2| fast0| (OCoLC)fst01026447;

650 7 a| Proteoglycans.2| fast0| (OCoLC)fst01079778;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Capehart, Anthony A.,e| degree supervisor.?| UNAUTHORIZED;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/12773;

949 a| Click on web addressw| asish| joyner101;

949 a| Click on web addressw| asish| hsl111;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 1055784;

999 a| ASK AT SPECIAL COLLECTIONS DESKw| ASISc| 1i| MQ1858437l| JSCJTHDm| JOYNERr| Ys| Yt| JSCJTHDu| 9/19/2006x| ETDz| JSPECCOLLo| .STAFF. 0;

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Versican expression during skeletal/joint morphogenesis and patterning of muscle and nerve in the embryonic mouse limb / by Holly Elizabeth Snow.

Author/creator	Snow, Holly Elizabeth author.
Other author	East Carolina University. Department of Biology.
Other author	Capehart, Anthony A., degree supervisor.
Format	Theses and dissertations
Production	2004.
Description	57 leaves : illustrations (some color) ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Extremities (Anatomy) Proteoglycans. Morphogenesis. Mice as laboratory animals. Extremities. Proteoglycans.

Summary	Versican, an extracellular matrix proteoglycan, has been implicated in limb skeletogenesis and is localized in mesenchymal precartilage condensations. However, studies have not investigated spatial and temporal expression of versican and its relationship to muscle and nerve patterning during mammalian limb development. The heart defect {hdf) transgenic mouse has a lacZ reporter construct within the versican gene {hdf transgene), which provides a novel model by which to study the role of versican in limb development. In addition, histochemical localization of the lacZ reporter is a simple method for localization of transgene expression, Hdf transgene expression in whole mount heterozygous embryos and localization of versican relative to cartilage, muscle, and nerve tissues in paraffin-embedded limb sections of wild-type embryos from 10.5-14 dpc were evaluated by lacZ histochemistry and immunohistochemistry. Versican was localized within precartilage condensations of mesenchyme and nascent cartilage nodules, with expression diminishing upon maturation of the cartilage model. Interestingly, versican expression remained strong in developing synovial joint interzones, suggesting a potential function for versican in joint morphogenesis. Isolated myoblasts, incipient skeletal muscle masses, and neurites were absent in areas of strong versican expression within the developing limb. Versican expressing tissues may reserve space for the future limb skeleton and developing joints and may aid in patterning of muscle and nerve by deterring muscle migration and innervation into these regions.
General note	Presented to the faculty of the Department of Biology.
General note	Advisor: Anthony A. Capehart
Dissertation note	M.S. East Carolina University 2004
Bibliography note	Includes bibliographical references (leaves 46-57).
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

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Versican expression during skeletal/joint morphogenesis and patterning of muscle and nerve in the embryonic mouse limb / by Holly Elizabeth Snow.

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