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LEADER 04599ctm 2200553 i 4500
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ocm39487095
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OCoLC
005
20230725093301.0
008
980715s1997 xx a bm 000 0 eng d
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a| (Sirsi) o39487095
035
a| (OCoLC)39487095
040
a| ERE
b| eng
e| rda
c| ERE
d| OCLCQ
d| OCLCF
d| OCLCO
d| OCLCQ
d| OCLCO
d| OCL
d| OCLCO
d| OCLCQ
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049
a| EREE
050
4
a| QR186
b| .A76 1997
100
1
a| Armacost, J. L.,
e| author.
?| UNAUTHORIZED
245
1
0
a| Characteristics of the immune response to type III pneumococcal polysaccharide induced in BALB/c mice through the use of immunostimulating complexes /
c| by J.L. Armacost.
264
0
c| 1997.
300
a| 99 leaves :
b| illustrations ;
c| 28 cm
336
a| text
b| txt
2| rdacontent
337
a| unmediated
b| n
2| rdamedia
338
a| volume
b| nc
2| rdacarrier
502
b| M.S.
c| East Carolina University
d| 1997
500
a| Submitted to the faculty of the Department of Biology.
500
a| Advisor: A. Mason Smith
520
3
a| This research was designed to study the immune response in BALB/c mice to a type III pneumoccocal capsular polysaccharide-bovine serum albumin (BSA) conjugate incorporated in immunostimulating complexes (ISCOMs). The study included the distribution of the immunogen via different routes while monitoring both the systemic and mucosal responses. This work was based on the original hypothesis that a polysaccharide-carrier protein conjugate incorporated into ISCOMs would (1) induce an anamnestic response consisting primarily of IgG antibodies when delivered subcutaneously (s.q.) and intraperitoneally (i.p ), and (2) generate an IgA response when given orally to BALB/c mice. The humoral immune responses were monitored in serum and intestinal gavages by ELISA and PHA. The quantification of splenocytes producing antibody specific for type III polysaccharide was done by ELISPOT. Peyer's patch cells were characterized after staining thin sections with hematoxylin and eosin. In preliminary studies, type III pneumococcal polysaccharide was conjugated with BSA using two different chemical reagents; 4-(maleimidomethyl) cyclohexane 1- Carboxyl hydrazide ( M2C2H) and cyanuric chloride (C3CI3N3). The presence of the ISCOMs structure was verified by electron microscopy, and its incorporated conjugate by carbohydrate assay and protein assay. ISCOMs injected i.p. and s.q. induced both an IgM and IgG systemic response. The IgG response was shown to be continuous over several weeks. Data obtained by ELISPOT indicated that the spleen was a major source of antibodies produced. Therefore, presented evidence supports the induction of an anamnestic response when the type III polysaccharide was given as part of the ISCOM vehicle. Oral feeding of the polysaccharide in the ISCOM vehicle did not produce secretory IgA in the intestine as measured in this study. However, oral feeding did produce a systemic IgG response and significant changes in Peyer's patch cell populations. These findings are discussed along with the importance of ISCOMs as a means of inducing protective immunity against some common bacterial polysaccharides.
504
a| Includes bibliographical references (leaves 78-86).
650
0
a| Immune response.
=| ^A1311
650
0
a| Serum albumin.
=| ^A14954
650
0
a| Mice as laboratory animals.
=| ^A848
650
2
a| Serum Albumin.
0| (DNLM)D012709
=| ^A950563
655
2
a| Academic Dissertation.
0| (DNLM)D019478
?| UNAUTHORIZED
650
7
a| Immune response.
2| fast
0| (OCoLC)fst00967867
650
7
a| Mice as laboratory animals.
2| fast
0| (OCoLC)fst01019388
650
7
a| Serum albumin.
2| fast
0| (OCoLC)fst01113386
655
7
a| Academic theses.
2| fast
0| (OCoLC)fst01726453
655
7
a| Academic theses.
2| lcgft
655
7
a| Thèses et écrits académiques.
2| rvmgf
0| (CaQQLa)RVMGF-000001173
700
1
a| Smith, A. Mason,
e| degree supervisor.
?| UNAUTHORIZED
710
2
a| East Carolina University.
b| Department of Biology.
=| ^A637467
856
4
1
z| Access via ScholarShip
u| http://hdl.handle.net/10342/11466
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