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LEADER 05027cam 2200637Ii 4500
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ocn919448438
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OCoLC
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20160202100012.0
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150831s2015 ncua ob 000 0 eng d
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a| (OCoLC)919448438
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043
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a| EREE
090
a| QR111
100
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a| Thigpen, Joshua C.,
e| author.
?| UNAUTHORIZED
245
1
0
a| Effects of nutrient availability and disturbance on the composition and diversity of soil microorganisms /
c| by Joshua C. Thigpen.
246
1
i| title from abstract page
a| Effects of nutrient availability and disturbance on the composition and diversity of soil microbial communities
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a| [Greenville, N.C.] :
b| [East Carolina University],
c| 2015.
300
a| 87 pages :
b| illustrations (some color)
336
a| text
b| txt
2| rdacontent
337
a| computer
b| c
2| rdamedia
338
a| online resource
b| cr
2| rdacarrier
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a| text file
b| PDF
c| 735.8Kb
2| rda
538
a| System requirements: Adobe Reader.
538
a| Mode of access: World Wide Web.
502
b| M.S.
c| East Carolina University
d| 2015.
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a| Presented to the faculty of the Department of Biology.
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a| Advisors: Carol Goodwillie and Ariane L. Peralta.
500
a| Title from PDF t.p. (viewed October 8, 2015).
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3
a| Determining how factors such as disturbance and nutrient availability affect species diversity in a community has been a major goal of community ecology. The purpose of this study was to look at how species diversity and composition of soil bacterial communities are affected by nutrient addition and disturbance. I characterized soil microbial communities at the long-term ecological research site at the West Research Campus (WRC) located in Pitt County, NC. Briefly, DNA extracted from soils was analyzed using amplicon sequencing of the 16S ribosomal RNA gene. The Illumina Platform was used to sequence the bacterial DNA from each sample, and the Mothur Pipeline was used to analyze the DNA sequences. I hypothesized that changes in nutrient availability and disturbance would impact soil microbial community composition and diversity through direct and indirect effects mediated by plant-soil interactions. My research complemented previous work carried out in the WRC determining the effects of nutrient addition and disturbance on plant communities. Analysis of 2013 plant data showed that mowing increased plant species richness, and fertilization decreased plant species richness significantly. The experimental treatments as well as the proximity of the blocks to a drainage ditch all had significant effects on plant community composition. Analysis of the microbial community data showed that both fertilization and mowing significantly increased mean species richness. Relative abundance microbial community composition varied significantly due to the proximity of the blocks to the ditch. Presence/absence microbial community analyses showed significant effects of the treatments, as well as ditch proximity on microbial composition differences. Also, unknown microbial communities showed significant variation of the communities due to the treatments. The results of the presence/absence analysis and the unknown microbial community analysis show the importance of rare taxa and unknown microbial communities to the differences in composition of our soil microbial communities. Analysis of the soil chemical and physical data showed very little variation due to the treatments. This study will contribute to our understanding of how both plant and soil bacterial community diversity are affected by anthropogenic nutrient addition and disturbances. Maintaining diversity is important for ecosystem stability and functioning.
504
a| Includes bibliographical references.
650
0
a| Soil microbiology
z| North Carolina
z| Pitt County.
=| ^A2102
650
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a| Biotic communities
z| North Carolina
z| Pitt County.
=| ^A28447
650
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a| Species diversity
z| North Carolina
z| Pitt County.
=| ^A282113
650
0
a| Nature
x| Effect of human beings on
z| North Carolina
z| Pitt County.
=| ^A5558
653
a| Ecology
653
a| Microbiology
653
a| Botany
653
a| Composition
653
a| Diversity
653
a| Microbes
653
a| Plant
653
a| Soil
700
1
a| Goodwillie, Carol,
e| degree supervisor.
=| ^A1170785
700
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a| Peralta, Ariane L.,
e| degree supervisor.
?| UNAUTHORIZED
710
2
a| East Carolina University.
b| Department of Biology.
=| ^A637467
856
4
0
z| Access via ScholarShip
u| http://hdl.handle.net/10342/5040
949
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994
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r| Y
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t| JNE3ETD
u| 8/31/2015
x| ETD
z| JERESOURCE
999
a| CLICK ON WEB ADDRESS
w| ASIS
c| 1
i| 3808181-2001
l| HSLELEC
m| HSL
r| Y
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t| HEETD
u| 8/31/2015
x| ETD
z| HERESOURCE