ECU Libraries Catalog
Librarian View
LEADER 05416cam 2200697Ii 4500
001
ocn869897630
003
OCoLC
005
20141212062930.0
006
m o d
007
cr bn|||||||||
008
140205s2013 ncua ob 000 0 eng d
035
a| (Sirsi) o869897630
035
a| (OCoLC)869897630
040
a| ERE
e| rda
c| ERE
d| ERE
d| UtOrBLW
049
a| EREE
100
1
a| Vidanapathirana, Achini Kushanthi,
e| author.
?| UNAUTHORIZED
245
1
0
a| Influence of carbon nanomaterial exposure on pro-constrictor mechanisms during pregnancy /
c| by Achini Kushanthi Vidanapathirana.
264
1
a| [Greenville, N.C.] :
b| [East Carolina University],
c| 2013.
300
a| 245 pages :
b| illustrations (some color)
336
a| text
b| txt
2| rdacontent
337
a| computer
b| c
2| rdamedia
338
a| online resource
b| cr
2| rdacarrier
347
a| text file
b| PDF
c| 6.99Mb
2| rda
490
1
a| ECU Brody School of Medicine thesis
538
a| System requirements: Adobe Reader.
538
a| Mode of access: World Wide Web.
502
b| Ph.D.
c| East Carolina University
d| 2013.
500
a| Presented to the faculty of the Department of Physiology.
500
a| Advisor: Christopher Wingard.
500
a| Title from PDF t.p. (viewed February 05, 2014).
520
3
a| Engineered carbon based nanoparticles (CNP) such as fullerenes and multiwalled carbon nanotubes (MWCNTs) are increasingly used in industries and in nanomedicine as a platform for drug delivery. Following environmental/occupational exposure through inhalation CNP are translocated to extra-pulmonary sites, raising concerns on their potential effects on vasculature particularly in vulnerable life-stages such as pregnancy. Distribution of intravenously delivered C60 in maternal organs varies between pregnant and non-pregnant life-stages, presumed to be associated with changes in reactivity of various vascular beds during pregnancy. The overall goal of this dissertation was to describe and elucidate intracellular mediators driving changes in contractility of vascular segments from three distinct vascular beds following exposure to CNP during pregnancy. In vitro experiments on aortic endothelial cells and wire myographic studies on vessel segments from Sprague Dawley rats exposed to C60/PVP (polyvinylpyrorrolidone formulated C60) or MWCNTs were used to identify changes in vascular tissue contractility and to determine the contribution of Rho-kinase pathway. Intravenous administration of C60/PVP increased contraction in the uterine artery, aorta and umbilical vein while pulmonary exposure to MWCNTs increased contraction in the uterine artery. These changes were confined to pregnancy, suggesting a higher susceptibility of the uterine vasculature to CNP mediated changes during pregnancy. Both in vitro and wire myographic studies suggested an increase in Rho-kinase activity with C60/PVP exposure (but not with MWCNT exposure), suggesting that C60/PVP mediated increase in contractility may be driven by a common pathway involving Rho-kinase via activation of endothelium and/or altering signaling within smooth muscle. MWCNT exposure induced contractions may be mediated by inflammatory mechanisms as suggested by the increased inflammatory endothelial markers. Our comparisons with naive rats and untreated endothelial cells indicated a critical role played by the dispersion medium used for CNP delivery in determining contractile effects. These CNP exposures may be detrimental to fetal growth resulting in the observed reduction of fetal weight gain following acute exposure to both CNP. The conclusions drawn from this work contribute to both nanomedicine and nanotoxicology, focusing on safe applications of nanotechnology. It also widens our understanding on the life-stage related changes in susceptibility to environmental insults.
504
a| Includes bibliographical references.
650
2
a| Nanoparticles.
=| ^A1098923
650
2
a| Nanostructures.
=| ^A946737
650
2
a| Nanotubes, Carbon
x| adverse effects.
=| ^A1123491
650
2
a| Nanomedicine
x| trends.
=| ^A1195274
650
2
a| Drug Delivery Systems
x| adverse effects.
=| ^A1195252
650
2
a| Inhalation
x| physiology.
=| ^A1195171
650
2
a| Lung
x| blood supply.
=| ^A946842
650
2
a| Pregnancy.
=| ^A918777
650
2
a| rho-Associated Kinases
x| physiology.
=| ^A1195278
650
2
a| Models, Animal.
=| ^A935493
650
2
a| Rats, Sprague-Dawley.
=| ^A1195257
650
2
a| Nanotechnology
x| methods.
=| ^A943321
650
2
a| Occupational Health
x| standards.
=| ^A1195248
650
2
a| Environmental Health
x| trends.
=| ^A1001409
653
a| Physiology
653
a| Toxicology
653
a| Occupational health
700
1
a| Wingard, Christopher J.,
e| degree supervisor.
?| UNAUTHORIZED
710
2
a| East Carolina University.
b| Department of Physiology.
?| UNAUTHORIZED
830
0
a| ECU Brody School of Medicine thesis.
=| ^A964744
856
4
0
z| Access via ScholarShip
u| http://hdl.handle.net/10342/4346
949
a| Click on web address
w| ASIS
h| JOYNER101
949
a| Click on web address
w| ASIS
h| HSL111
994
a| C0
b| ERE
096
a| QT 36.5
596
a| 1 4
998
a| 3422708
999
a| CLICK ON WEB ADDRESS
w| ASIS
c| 1
i| 3422708-1001
l| JNET
m| JOYNER
r| Y
s| Y
t| JNE3ETD
u| 2/5/2014
x| ETD
z| JERESOURCE
999
a| CLICK ON WEB ADDRESS
w| ASIS
c| 1
i| 3422708-2001
l| HSLELEC
m| HSL
r| Y
s| Y
t| HEETD
u| 2/5/2014
x| ETD
z| HERESOURCE